NorClone provides site-directed mutagenesis service quickly and economically to introduce single or multiple mutations (substitutions, insertions, deletions, or truncations) into existing DNA sequence. These services include:
 

   1) Design, synthesis, and purification of oligo nucleotide primers.

   2) Mutagenesis reaction, enzyme digestion and/or ligation.

   3) Bacterial transformation.

   4) Plasmid isolation.

   5) Confirmation of the desired mutation by DNA sequencing.

You provide:

   1) 2~5 µg of the target gene in a plasmid.

   2) Sequence of the target gene with a list of the bases to be mutated.

You will receive:

   1) 2~5 µg of mutant plasmid.

   2) Stab culture of E. coli carrying the mutant plasmid.

   3) Sequencing chromatogram and text files.

Turnaround Time:

   Approximately 1~2 weeks.

Prices for mutagenesis from customer-provided Templates:

Please contact us for a detailed quote or for more information.